Fig. 2
From: Production of (S)-2-aminobutyric acid and (S)-2-aminobutanol in Saccharomyces cerevisiae
Intracellular accumulation of (S)-2-aminobutyric acid in S. cerevisiae expressing different enzyme combinations. a Either two different threonine deaminases (orange bars ScCHA1, green bars EcILVa) for the first step of the (S)-2-aminobutyric acid pathway were expressed in combination with either one of six heterologous enzymes for the second step of the pathway. Yeasts that did not express the pathway enzymes were included as control. b EcGDH’ for the second step of the pathway was expressed in combination with either one of five different threonine deaminases. Diamonds indicate OD600 after 24 h of growth (all data: mean ± SD, n = 3)