Fig. 2
From: Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies
Analyzing transcriptional regulation on yeast promoters using the constitutive system. a Log-scaled mean fluorescense intensity for 12 yeast promoters involved in glycolysis and mevalonate metabolism is shown. Activation with MCP-VPR (dark grey) and repression with PCP-Mxi1 (light grey) is shown next to ‘no gRNA’ controls (black; dCas9 expressed) for best performing scRNAs out of 88 for each promoter. Asterisks indicate regulation that resulted in significantly altered expression profiles relative to controls (*p < 0.05). MFI values are shown as mean ± S.D. from three (n = 3) biological replicate experiments. b Best performing scRNAs for repression (light grey) and for activation (dark grey) are the same as presented in a. Relative MFI (deviation from ‘no gRNA’ control strains Sc-23 to Sc-34 expressing dCas9) conferred by 88 scRNAs (in strains Sc-35 to Sc-122) tethering dCas9 and PCP-Mxi1 or MCP-VPR on a total of 12 promoters is illustrated in black relative to PAM distance (nt) from TSS+1