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Table 3 Yields of recombinant GFP, HRP and A2aR can be improved in the high-yielding yeast strains

From: Constitutively-stressed yeast strains are high-yielding for recombinant Fps1: implications for the translational regulation of an aquaporin

Yeast strain

GFP yield (AU)

HRP yield (U/mL)

A2aR yield (pmol/mg)

Wild-type (BY4741)

1.0 (0.2)

5.9 (0.7)

7.4 (0.7)

yTHCBMS1

0.9 (0.2)

28.1 (4.0)

–

yTHCBMS1 + 0.5 µg/mL doxycycline

1.3 (0.1)

–

9.6 (1.3)

yTHCBMS1 + 10 µg/mL doxycycline

4.7 (0.8)

–

16.1 (1.7)

srb5Δ

1.6 (0.3)

–

–

gcn5Δ

1.5 (0.4)

–

–

spt3Δ

0.8 (0.1)

16.0 (2.0)

–

  1. Yeast cells were transformed with a plasmid expressing green fluorescent protein (GFP; Additional file 1: Figure S7), horseradish peroxidase (HRP; Additional file 1: Figure S8) or the adenosine A2a receptor (A2aR; Additional file 1: Figure S9). Single transformants were cultured in shake flasks in 2× CBS lacking histidine (to maintain the plasmid). GFP yields were determined by fluorimetry of the culture supernatant as previously described [6], but with cells harvested from 50 mL cultures at A600 = 4. They are reported as arbitrary units (AU) normalised to the value for wild-type cultures. HRP yields are reported in U/mL 24 h post-induction, where one unit of HRP decomposes 1 µmol of peroxide/min at 25 °C. Data for A2aR were published previously [6] and are reported as Bmax values (pmol/mg membrane). All data shown are the mean of biologically-independent, triplicate determinations; the standard error of the mean (SEM) is shown in parentheses
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Microbial Cell Factories

ISSN: 1475-2859

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