Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1

Table 4 qRT-PCR evaluation of the ED pathway gene expression in MM medium containing glucose or gluconate as sole carbon source

Gene	Annotation	Carbon source	Ct^a	ΔCt^b	FoldChange
ro02362	Probable gluconokinase	RM	30.254	–
		MMGln	25.539	5.135	35.14
		MMGls	26.316	4.335	20.18
ro02367	KHG/KDPG aldolase	RM	24.194	–
		MMGln	20.708	3.378	10.39
		MMGls	21.324	2.762	6.78
ro02369	Glucose-6-P 1-dehydrogenase	RM	24.084	–
		MMGln	20.857	3.129	8.75
		MMGls	22.018	2.478	5.57
ro00588	Cold shock protein	RM	21.195	–
		MMGln	21.086	−0.109	0.92
		MMGls	21.596	0.401	1.32

^a Ct is the cycle threshold or number of cycles requires for the fluorescence signal to cross the threshold. The Cts shown are the mean of three experiments
^b ΔCt = Ct (MM) − Ct (RM)

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