High-level extracellular protein production in Bacillus subtilis using an optimized dual-promoter expression system

Table 2 Plasmids

Plasmid	Description	Reference
pNCMO2/pulA-d2	Brevibacillus choshinensis–E. coli shuttle vector, Amp^r (E. coli), Ner^r (Brevibacillus choshinensis), pullulanase gene	[22]
pHY300PLK	B. subtilis–E. coli shuttle expression vector, Amp^r (E. coli), Tet^r (B. subtilis and E. coli)	Takara
pHYCGT1	B. subtilis–E. coli shuttle expression vector, Amp^r (E. coli), Tet^r (B. subtilis and E. coli), amylase promoter P_amyQ and signal peptide amyQ from Bacillus amyloliquefaciens, β-CGTase gene.	[28]
pET-20b(+)/cgt	E. coli gene expression vector, Amp^r, α-CGTase gene	[29]

pHYCGT1 derivative	Promoter	Signal peptide	Reporter gene	Reference
pHYCGT2	P_srf	amyQ	β-CGTase gene	This work
pHYCGT3	P_xyl′	amyQ	β-CGTase gene	This work
pHYCGT4	P_gsiB	amyQ	β-CGTase gene	This work
pHYCGT5	P_xyl	amyQ	β-CGTase gene	This work
pHYCGT6	P_HpaII	amyQ	β-CGTase gene	This work
pHYCGT7	P_amyQ′	amyQ′	β-CGTase gene	This work
pHYCGT8	P_aprE	aprE	β-CGTase gene	This work
pHYCGT9	P_nprE	nprE	β-CGTase gene	This work
pHYCGTd1	P_srf and P_amyQ′	amyQ′	β-CGTase gene	This work
pHYCGTd2	P_xyl′ and P_amyQ′	amyQ′	β-CGTase gene	This work
pHYCGTd3	P_gsiB and P_amyQ′	amyQ′	β-CGTase gene	This work
pHYCGTd4	P_HpaII and P_amyQ′	amyQ′	β-CGTase gene	This work
pHYCGTd5	P_amyQ′ and P_amyQ′	amyQ′	β-CGTase gene	This work
pHYCGTd6	P_nprE and P_amyQ′	amyQ′	β-CGTase gene	This work
pHYPUL1	P_amyQ	amyQ	Pullulanase gene	This work
pHYPUL7	P_amyQ′	amyQ′	Pullulanase gene	This work
pHYPULd4	P_HpaII and P_amyQ′	amyQ′	Pullulanase gene	This work
pHYαCGT1	P_amyQ	amyQ	α-CGTase gene	This work
pHYαCGT7	P_amyQ′	amyQ′	α-CGTase gene	This work
pHYαCGTd4	P_HpaII and P_amyQ′	amyQ′	α-CGTase gene	This work

ISSN: 1475-2859