Fig. 5
Extracellular expression of pullulanase and α-CGTase mediated by promoters PamyQ, PamyQ′ and PHpaII–PamyQ′. Extracellular enzyme activity profile (column) and growth curve (line chart) of pullulanase expression strains PUL1, PUL7 and PULd4 (a), and α-CGTase expression strains αCGT1, αCGT7 and αCGTd4 (b). SDS-PAGE analysis of protein expression at 48 h by these plasmid-containing strains (c) (P < 0.05)