Table 1 List of primers used in this study
Primer | Sequence | Use |
|---|---|---|
HYB-ClaI3′Hp4d5′ | CCCTACATCGATACGCGTGC | Hybrid promoter construction |
HYB-BstbI5′Hp4d3′ | CCTTCGAACGCACTTTTGCCCGTGATCAG | |
GATO_Amont_ClaI_for | CCCTGTTATCCCTAGAATCGAT | Verification of plasmid construction and insertion into the Y. lipolytica genome |
GATO_Aval_AvrII_rev | TTAGATACCACAGACACCCTAG | |
GATO_pTEF_BamHI_for | AACTCACACCCGAAGGATCC | |
GATO_HP4d_BamHI_for | GAACCCGAAACTAAGGATCC | |
YFP-pool-Fw | CACCATGGTGAGCAAGGGCGAGGAGC | Insertion of YFP gene into pENTR™/D-TOPO® |
YFP-pool-Rv | TTACTTGTACAGCTCGTCCATGCC | |
Amy-pool-Fw | CACCATGAAGCTGTCTACCATTCTG | Insertion of α-amylase gene into pENTR™/D-TOPO® |
Amy-pool-Rv | TCAAATCTTCTCCCAAATAGCG | |
1529BamHIcorrigéF | CCTTGTCAACTCACACCCGAAGGATCCATCACAAGTTTGTAC | Addition of a BamHI site close to the promoter in JMP1529 to obtain JMP3030 |
1529BglIIcorrigéR | TCTGGCTTTTAGTAAGCCAGATCTACGCGTTTACGCCCCGCC | |
1529BamHIcorrigéR | GTACAAACTTGTGATGGATCCTTCGGGTGTGAGTTGACAAGG | |
qPCR_XlnCF | CGAGCTGCCGATCCCAATGCC | qPCR related to the XlnC gene |
qPCR_XlnCR | GCTCCACCGCCTGCAGACA | |
qPCR_YALI0D08272F | AGGCCCAGTCCAAGCGAGGT | qPCR related to the actin gene |
qPCR_YALI0D08272R | TCGGTGAGCAGGACGGGGTG |