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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica

Fig. 6

Results of the vector-pool method when used to identify clones with enhanced YFP and α-amylase production. a Fluorescence patterns of the strains overexpressing YFP. The figure depicts the negative controls (i.e., the wild-type strain, Y2900, and yeast transformants grown on YNB), the positive controls (the 4 strains overexpressing YFP via pTEF), and the 54 strains obtained by the transformation of Y. lipolytica via the pool of vectors. The most interesting results are associated with clones E6, G10, and F8. b Starch consumption patterns for the strains overexpressing α-amylase. The figure depicts the negative controls (B2: yeast grown on YNB and C2: the wild-type strain, Y2900), the positive controls (D2, E2, F2, and G2: the 4 strains overexpressing α-amylase via the TEF promoter), and the 54 strains produced by the transformation of Y. lipolytica via the pool of vectors. Starch consumption was measured using iodine crystals

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Microbial Cell Factories

ISSN: 1475-2859

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