Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome

Table 1 Chi18H8 solubilization from IBs of E. coli BL21 Star™(DE3)/pET24b(+)::chi18H8 cells in acidic pH conditions

Solubilization agent	Concentration (mM)	Solubilization yield (%)	Specific activity (U/mg_Chi18H8)^a
HCl	10	93 ± 4.0	29.6 ± 1.2
HCl	100	20 ± 2.0	15.9 ± 0.9
Lactic acid	10	82 ± 3.5	40.7 ± 1.4
Lactic acid	100	60 ± 6.5	30.7 ± 1.3

Solubilization yield was estimated by densitometric analysis of proteins separated by SDS-PAGE. Enzyme activity was measured by fluorimetric assay on 4-MU-(GlcNAc)₂
^aValues represent the mean of three independent experiments (mean ± standard error)

ISSN: 1475-2859