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Table 1 Chi18H8 solubilization from IBs of E. coli BL21 Star™(DE3)/pET24b(+)::chi18H8 cells in acidic pH conditions

From: Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome

Solubilization agent Concentration (mM) Solubilization yield (%) Specific activity (U/mgChi18H8)a
HCl 10 93 ± 4.0 29.6 ± 1.2
100 20 ± 2.0 15.9 ± 0.9
Lactic acid 10 82 ± 3.5 40.7 ± 1.4
100 60 ± 6.5 30.7 ± 1.3
  1. Solubilization yield was estimated by densitometric analysis of proteins separated by SDS-PAGE. Enzyme activity was measured by fluorimetric assay on 4-MU-(GlcNAc)2
  2. aValues represent the mean of three independent experiments (mean ± standard error)