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Table 1 Summary production of recombinant glycoproteins in R. erythropolis

From: Rhodococcus erythropolis as a host for expression, secretion and glycosylation of Mycobacterium tuberculosis proteins

Purified proteina from R. erythropolis transformed with

Total purified protein (mg) from soluble extract

Total purified protein (mg) from insoluble fraction

Total purified protein (mg) from culture supernatant

pTip-QC1-apa b

0.355 ± 0.07

0.334 ± 0.06

0.269 ± 0.042

pNit-QC1-apa c

0.356 ± 0.09

0.349 ± 0.09

0.240 ± 0.06

pNit-QC1-pstS1 d

0.403 ± 0.04

0.399 ± 0.05

0.106 ± 0.012

pTip-QC1-lprG e

0.011 ± 0.002

0.019 ± 0.01

NA

pNit-QC1-lprG f

0.017 ± 0.004

0.016 ± 0.002

NA

  1. NA no applicable
  2. a From 50 ml culture (b0.717 ± 0.155, c0.663 ± 0.184, d0.642 ± 0.07, e0.585 ± 0.107, f0.517 ± 0.102 g wet weight)