Table 2 Description of plasmids used in this work
From: Human β-defensin-2 production from S. cerevisiae using the repressible MET17 promoter
Plasmid | Description |
|---|---|
pDB3936 | pSAC35, a 2 μm-derived yeast episomal expression vector with a truncated LEU2 [36] |
pDB4081 | Containing the expression cassette; PRB1 promoter, the mHSA/MFα1-leader leader, the coding sequence of rHA and the S. cerevisiae ADH1 transcription terminator |
pDB4146 | Containing the expression cassette; PRB1 promoter, the MFα1/plectasin leader, the coding sequence of hBD2 and the S. cerevisiae ADH1 transcription terminator |
pDB4351 | Containing the expression cassette; MET17 promoter, the MFα1/plectasin leader, the coding sequence of hBD2 and the S. cerevisiae ADH1 transcription terminator |
pDB4692 | Containing the expression cassette; MET17 promoter, the mHSA/MFα1-leader leader, the coding sequence of rHA and the S. cerevisiae ADH1 transcription terminator |