Fig. 2

Relative hBD2 and rHA productivity determined by UPLC-MS analysis. hBD2 and rHA were expressed using either the MET17 or the PRB1 promoter on 2 µm-based expression plasmids. Plasmids were transformed into S. cerevisiae strain DYB7 or DB1. The transformed yeast were inoculated at OD₆₀₀ = 0.15 into BMMD SFC without or with 1000 µM methionine and grown for 5 days, while culture samples were taken approximately every 24 h. Error bars indicate standard deviations (n = 3). a Expression of hBD2 from the MET17 promoter (pDB3936:GR:pDB4351) under non–repressing (0 µM methionine) and initially repressing conditions (1000 µM methionine) in DYB7. b Expression of hBD2 from the PRB1 promoter (pDB3936:GR:pDB4146) without methionine (0 µM) and with methionine in the media initially (1000 µM) in DYB7. c Expression of hBD2 from the MET17 promoter (pDB3936:GR:pDB4351) under non-repressing (0 µM methionine) and initially repressing conditions (1000 µM methionine) in DB1. d Expression of rHA from the MET17 promoter (pDB3936:GR:pDB4692) under non-repressing (0 µM methionine) and initially repressing conditions (1000 µM methionine) in DYB7