Fig. 3From: Recombinant production of the antibody fragment D1.3 scFv with different Bacillus strainsCultivation profile of recombinant B. megaterium MS941 secreting D1.3 scFv in microtiter plates and bioreactors. B. megaterium MS941 was transformed with plasmid pRBBm117 (P opt. xylA -sp lipA -d1.3scFv-his 6 ). Cultivation took place in microtiter plates (1250 μL culture volume) and stirred tank bioreactors (2 L culture volume) under aerobic conditions at 37 °C. All cultivation systems were inoculated with cell concentrations (cell dry weight) of 0.0223 g L−1 in minimal medium containing 10 mg L−1 of tetracycline. Recombinant D1.3 scFv secretion was induced by the addition of 5 g L−1 of xylose at cell concentrations of around 0.07 g L−1. Samples were taken at given time points to analyze cell dry weight [g L−1] and extracellular concentration of D1.3 scFv [mg L−1] using ELISA. Dissolved oxygen was measured online. Further analyses are shown in Additional file 1: Figure S1Back to article page