Fig. 3

Dissection of the phenotypic contributions of each individual mutation in the identified HAA1 mutant allele HAA1 ^C404T_G1447T (protein Haa1^{S135F_Truncated}). The effects of the modified proteins were characterized after allele swapping in strain CEN.PK113-7D, respectively. a Growth curves, b maximum specific growth rates and duration of latency phases, and c fraction of cells that resume proliferation are shown after shifting exponentially growing cells from medium without acetic acid to medium with 160 mM acetic acid at pH 4.5 are shown. The isogenic wild type CEN.PK113-7D (Haa1^WT) and the strain containing a second copy of the endogenous HAA1 expression cassette (Haa1^OE) were also included in these experiments. Mean values and standard deviations of at least three biological replicates are shown