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Table 1 Loci of pALG-transposon insertions in S. albus J1074 identified by rescue plasmid sequencing

From: Chromosomal position effect influences the heterologous expression of genes and biosynthetic gene clusters in Streptomyces albus J1074

M

Locus (XNR)

Gene function

GUS activity (U/mg)

St. dev. (U/mg)

Expression level (RPKM)

1

1302

Urease subunit α 1

8.2

0.6

1.9

2

1853

Bi-functional transferase/deacetylase

7.7

0.4

2.2

3

1880

Phosphatase

15.4

2.1

42.5

4

2035

Cons. hyp. prot./biosynthesis docking scaffold prot.

9.1

1.3

5.7

5

2089

Integral membrane protein

8.1

0.1

24.7

6

2358

Reg. prot./MerR-family transcriptional reg.

11.8

1.1

22.7

7

2599

Predicted protein/adenylate cyclase

17.3

1.8

3.5

8

2683

Beta-lactamase

3.4

1.7

31.7

9

2883

TetR-family transcriptional regulator

6.2

1.2

6.0

10

2981

Integral membrane protein

3.5

0.5

102.6

11

3133

Peptidase C14 caspase catalytic subunit

3.3

0.1

97.1

12

3300

Conserved hypothetical protein

2.7

0.9

2.3

13

3826

Conserved hypothetical protein/DNA-binding prot.

9.9

1.1

27.5

14

4204/5

IGR (intergenic region) btw. two predicted proteins

8.6

1.1

25.4

15

4675/6

IGR btw. cons. hyp. protein and phospholipase

14.7

1.5

19.6

16

5073/4

IGR btw. transmembr. transporter and integral membr. prot.

8.8

1.3

3.5

17

5168

Glycerol kinase 1/2

7.8

1.0

27.9

18

5663

Succinate dehydrogenase flavoprotein subunit

8.0

1.3

29.9