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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Chromosomal position effect influences the heterologous expression of genes and biosynthetic gene clusters in Streptomyces albus J1074

Fig. 1

The map of the plasmid containing gus(a) in the transposon, pALG. Plasmids contains following features: himar1(a)—synthetic transposase gene, under control of p1—tipAp, thiostrepton inducible promoter; gus(a)—reporter gene of glucuronidase; p2—ermEp1, promoter 1 of erythromycin resistance gene; tfd terminator of fd-phage, pSG5rep actinomycetes temperature-sensitive replicon; aac(3)IV apramycin resistance marker, hph hygromycin resistance marker, ITR inverted terminal repeats; R6Kori origin for rescue cloning

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