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Table 4 Different growth mediums used in the present study

From: The ACEII recombinant Trichoderma reesei QM9414 strains with enhanced xylanase production and its applications in production of xylitol from tree barks

Growth medium

Composition (g/l)

pH

Potato dextrose agar

Potato dextrose 15 g, sugar 20 g, KH2PO4 3 g, MgSO4·7H2O 2 g, agar 15 g

pH is maintained between the range of 5.6–5.8

Yeast glucose medium

Yeast extract 5 g, glucose 20 g, microelement solution of 400 μl. The microelement solution consists of EDTA 10 g, ZnSO4·7H2O 4.4 g, MnCl2 1.01 g, CoCl2·6H2O 0.32 g, CuSO4·5H2O 0.315 g, (NH4)6Mo7O24·5H2O 0.22 g, CaCl2·2H2O 1.47 g, FeSO4·7H2O 1 g, dissolved in 900 ml double distilled water

The pH is adjusted to 6.0 using 1 M NaOH, once all the constituents are dissolved add 1 M HCl to adjust the pH to 4.0, later the solution is made up to 1000 ml using dd-H2O

Mandel and andreotti (MA-medium)

KH2PO4 2 g, (NH4)2SO4 1.4 g, Urea 0.3 g, FeSO4·7H2O 0.005 g, MnSO4·H2O 0.0016 g, ZnSO4·7H2O 0.0014 g, CoCl2 0.002 g, MgSO4·7H2O 0.3 g, CaCl2 0.3 g, peptone 0.75 g, glucose 10 g

pH is maintained between the range of 4.8–5.0

2% glycerol-MA medium

Glycerol 10 g in 500 ml of MA medium

pH is maintained between the range of 5.4–5.8

2% bark-MA sugar-free medium

Bark powder 10 g dissolved in 500 ml of MA medium (composition is same as mentioned above but without glucose)

pH is maintained between the range of 5.4–5.8

2% bark-MA glucose medium

Bark powder 10 g dissolved in 500 ml of MA medium with glucose 10 g

pH is maintained between the range of 5.4–5.8

2% bark-MA xylose medium

Bark powder 10 g dissolved in 500 ml of MA medium with xylose 10 g (glucose is replaced with xylose)

pH is maintained between the range of 5.4–5.8

Minimal salt medium (MSM) consists

Glucose 20 g, (NH4)2SO4 5 g, KH2PO4 15 g, MgSO4·7H2O 0.6 g, CaCl2 0.6 g, FeSO4·7H2O 0.005 g, MnSO4·H2O 0.0016 g, ZnSO4·7H2O 0.0014 g, CoCl2 0.002 g

pH between the range of 5.0–5.5

LB-medium

Tryptone 10 g, yeast extract 5 g, NaCl 5 g

pH is adjusted to 7.0