Growth medium | Composition (g/l) | pH |
---|---|---|
Potato dextrose agar | Potato dextrose 15 g, sugar 20 g, KH2PO4 3 g, MgSO4·7H2O 2 g, agar 15 g | pH is maintained between the range of 5.6–5.8 |
Yeast glucose medium | Yeast extract 5 g, glucose 20 g, microelement solution of 400 μl. The microelement solution consists of EDTA 10 g, ZnSO4·7H2O 4.4 g, MnCl2 1.01 g, CoCl2·6H2O 0.32 g, CuSO4·5H2O 0.315 g, (NH4)6Mo7O24·5H2O 0.22 g, CaCl2·2H2O 1.47 g, FeSO4·7H2O 1 g, dissolved in 900 ml double distilled water | The pH is adjusted to 6.0 using 1 M NaOH, once all the constituents are dissolved add 1 M HCl to adjust the pH to 4.0, later the solution is made up to 1000 ml using dd-H2O |
Mandel and andreotti (MA-medium) | KH2PO4 2 g, (NH4)2SO4 1.4 g, Urea 0.3 g, FeSO4·7H2O 0.005 g, MnSO4·H2O 0.0016 g, ZnSO4·7H2O 0.0014 g, CoCl2 0.002 g, MgSO4·7H2O 0.3 g, CaCl2 0.3 g, peptone 0.75 g, glucose 10 g | pH is maintained between the range of 4.8–5.0 |
2% glycerol-MA medium | Glycerol 10 g in 500 ml of MA medium | pH is maintained between the range of 5.4–5.8 |
2% bark-MA sugar-free medium | Bark powder 10 g dissolved in 500 ml of MA medium (composition is same as mentioned above but without glucose) | pH is maintained between the range of 5.4–5.8 |
2% bark-MA glucose medium | Bark powder 10 g dissolved in 500 ml of MA medium with glucose 10 g | pH is maintained between the range of 5.4–5.8 |
2% bark-MA xylose medium | Bark powder 10 g dissolved in 500 ml of MA medium with xylose 10 g (glucose is replaced with xylose) | pH is maintained between the range of 5.4–5.8 |
Minimal salt medium (MSM) consists | Glucose 20 g, (NH4)2SO4 5 g, KH2PO4 15 g, MgSO4·7H2O 0.6 g, CaCl2 0.6 g, FeSO4·7H2O 0.005 g, MnSO4·H2O 0.0016 g, ZnSO4·7H2O 0.0014 g, CoCl2 0.002 g | pH between the range of 5.0–5.5 |
LB-medium | Tryptone 10Â g, yeast extract 5Â g, NaCl 5Â g | pH is adjusted to 7.0 |