Table 2 Frequency of the correct gene deletion with and without CRISPR/Cas9
| Â | Protein ID | Screened | Correct | Frequency (%) |
|---|---|---|---|---|
No CRISPR/Cas9 | 39114 | 30 | 2 | 6.7 |
1090836 | 30 | 1 | 3.3 | |
1117792 | 30 | 13 | 43.3 | |
1141260 | 30 | 0 | 0.0 | |
1121140 | 60 | 1 | 1.7 | |
CRISPR/Cas9Â +Â in vitro sgRNA | 1090836 | 40 | 11 | 27.5 |
1117792 | 8 | 8 | 100 | |
1141260 | 8 | 8 | 100 | |
1121140 | 8 | 3 | 37.5 | |
1146483 | 8 | 7 | 87.5 | |
1170646 | 8 | 5 | 62.5 |