Table 1 Oligonucleotide primers and probes used for ddPCR
From: Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
Target | Genbank ID | Amplicon length | Primer/probe sequence | Tm/ °C | Length/bp |
|---|---|---|---|---|---|
cysG (E. coli genome) | NJ74_RS16775 | 127 | 5′-AGCCATTACTGAAACGACC-3′ | 60.16 | 19 |
[46] | 5′-GCTGAATTTGTTGCAGTCC-3′ | 60.16 | 19 | ||
5′-FAM-ACCAACCAGCACCACTTCACCG-BHQ-1-3′ | 69.99 | 22 | |||
oriT (SEVA plasmids) | JX560321.2 | 104 | 5′-CAGGTGCGAATAAGGGAC-3′ | 60.25 | 18 |
[1] | 5′-GTAGACTTTCCTTGGTGTATCC-3′ | 60.33 | 22 | ||
5′-HEX-CCTATCCTGCCCGGCTGACG-BHQ-1-3′ | 69.64 | 20 |