Skip to main content

Table 1 Oligonucleotide primers and probes used for ddPCR

From: Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR

Target Genbank ID Amplicon length Primer/probe sequence Tm/ °C Length/bp
cysG (E. coli genome) NJ74_RS16775 127 5′-AGCCATTACTGAAACGACC-3′ 60.16 19
[46]   5′-GCTGAATTTGTTGCAGTCC-3′ 60.16 19
   5′-FAM-ACCAACCAGCACCACTTCACCG-BHQ-1-3′ 69.99 22
oriT (SEVA plasmids) JX560321.2 104 5′-CAGGTGCGAATAAGGGAC-3′ 60.25 18
[1]   5′-GTAGACTTTCCTTGGTGTATCC-3′ 60.33 22
   5′-HEX-CCTATCCTGCCCGGCTGACG-BHQ-1-3′ 69.64 20