Fig. 4
From: Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
Average plasmid copy number (PCN) of p2X4-AEB vectors in E. coli. a Absolute concentration of the gDNA and pDNA marker genes cysG and oriT as determined by Droplet Digital PCR. Representative samples were taken from a batch cultivation for 24 h with induction by IPTG after 2 h. Grey dots single measurements, colored dots mean and standard deviation of four replicates. b PCN calculated as the ratio of pDNA (oriT) concentration and gDNA (cysG) concentration. Bars represent mean and standard deviation