Fig. 1
From: Proteins adopt functionally active conformations after type III secretion
Secreted proteins adopt functional conformations. Activity or ELISA signal is given for samples analyzed from the culture supernatant. Genetic modifications described are with respect to the mature native protein sequence of the POI in the fusion. All proteins are of the format SptP-POI-2xFLAG-6xHIS. Results are plotted for the POIs. a Beta-lactamase (Bla). b Alkaline phosphatase (PhoA). c Single chain variable fragment against anthrax protective antigen (14B7*). The mean is plotted from three biological replicate experiments and the error bars represent one standard deviation. Western blots are representative of the samples analyzed in the functional assays