Fig. 2

Agarose gel electrophoresis of colony PCR for poxb editing. a poxb gene knock out with 513 bp deletion: lanes 1,2 and 8 were control group samples, others were experimental group samples. Colony PCR products of edited poxb gene were 1008 bp, and original were 1521 bp. b poxb replacement by rfp: lanes 1 and 7 were control groups, others were experimental groups. Colony PCR products of edited poxb gene were 1823 bp, and original were 1521 bp. c poxb disruptions with homologous arms of 41 bp with both RecA and Red recombinases: all the bands were experimental group samples. Colony PCR products of edited poxb gene were 1008p