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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Siropins, novel serine protease inhibitors from gut microbiota acting on human proteases involved in inflammatory bowel diseases

Fig. 6

Progress curves for proteases inhibition by Siropins. a and c HNE inhibition by Siropin 1 (a) and Siropin 2 (c). b and d PR3 inhibition by Siropin 1 (b) and Siropin 2 (d). Constant concentrations of HNE or PR3 were used with various amount of Siropin, the change in the absorbance was then performed at 37 °C. Used concentration of Siropin (µM) are shown beside each progress curve. Obtained k obs rate were plotted against the Siropin concentration. The apparent second-order association constant k’ app was calculated as the slope of the fitted linear curve and then corrected to determine the second-order association constant k a

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Microbial Cell Factories

ISSN: 1475-2859

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