Fig. 2From: The effect of protein acetylation on the formation and processing of inclusion bodies and endogenous protein aggregates in Escherichia coli cellsIBs from wt and ΔcobB cells contain acetylated VP1GFP. IBs were isolated after 24 h of IPTG induction from the bacteria cultivated, as described in the legend to Fig. 1. The whole-cell extracts and purified IBs were submitted to SDS-PAGE and immunodetection using anti-GFP (Sigma) and anti-acetyl lysine antibodies (Abcam). IBs isolated from equal amounts of cells were loaded onto the gelBack to article page