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Fig. 2 | Microbial Cell Factories

Fig. 2

From: The effect of protein acetylation on the formation and processing of inclusion bodies and endogenous protein aggregates in Escherichia coli cells

Fig. 2

IBs from wt and ΔcobB cells contain acetylated VP1GFP. IBs were isolated after 24 h of IPTG induction from the bacteria cultivated, as described in the legend to Fig. 1. The whole-cell extracts and purified IBs were submitted to SDS-PAGE and immunodetection using anti-GFP (Sigma) and anti-acetyl lysine antibodies (Abcam). IBs isolated from equal amounts of cells were loaded onto the gel

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