Fig. 2

Microscopic analysis of the Synechococcus sp. PCC 7002 negative control and B-PER treated cells stained with the nucleic acid stain SYTOX Green. Bright field images of the negative control (a) and the permeabilized cells (b). Fluorescence imaging of SYTOX GREEN upon excitation at 488 nm and emission detection between 515 and 535 nm for the negative control (c) and the permeabilized cells (d). The fluorescence imaging of Chl a upon excitation at 573 nm and emission detection between 590 and 640 nm for the negative control (e) and the permeabilized cells (f). The same setting for imaging was applied for imaging of the control and the B-PER treated cells. At least three independent preparations were analyzed and representative images are shown here