Carbonyl reductase identification and development of whole-cell biotransformation for highly efficient synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol

Table 2 The effect of temperature and pH on asymmetric (R)-3,5-BTPE synthesis by recombinant E.coli cells co-expressing LkCR and BsGDH

Reaction conditions: 300 mM substrate, 450 mM glucose, 250 g/L wet cells, and incubation for 30 h with shaking at 220 rpm
^aReaction was performed in 100 mM PBS buffer (pH 7.0)
^bTemperature was 28 °C; pH 4.0–6.0 (0.2 M, NaAc–HAc), pH 6.0–7.0 (0.1 M Na₂HPO₄–NaH₂PO₄)

ISSN: 1475-2859