Fig. 3
From: A novel methanol-free Pichia pastoris system for recombinant protein expression
The Δdak strain has the potential to be developed to a novel methanol-free expression system. Concentrations of carbon sources were shown in brackets by the mass/volume percentage. M methanol; G glycerol; D glucose; DHA dihydroxyacetone. a Growth curves of the Δdak strain under different DHA concentrations. b Colorimetrical assay showing Aox activity of DHA cultured Δdak strain. Time points indicate cell growth hours in DHA. Aox activity was not detected in glucose cultured Δdak strain. Higher Aox activity corresponds to deeper red color in the colorimetrical assay. c Q-PCR comparing gene transcription levels in WT and Δdak strains grown on DHA. Folds were calculated towards the glycerol cultured WT strain. d Western blot showing Aox protein levels in DHA cultured Δdak strain. WT strains grown on methanol, glycerol and DHA serve as controls