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Table 2 Plasmids and S. cerevisiae strains used in this study

From: Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae

  Description Source
Plasmid
pY26TEF-GPD (pY26) URA3/2μ yeast shuttle vector containing GPD1 promoter [37]
pY26-RHO1 RHO1 expressed from GPD1 promoter in pY26TEF-GPD This study
pY26-FKS2 FKS2 expressed from GPD1 promoter in pY26TEF-GPD This study
pRS424TEF TRP1/2μ yeast shuttle vector containing TEF1 promoter [38]
pRS424TEF-LYZ hLYZ expressed from TEF1 promoter in pRS424TEF This study
pRS424TEF-LYZ(R59S) hLYZ(R59S) expressed from TEF1 promoter in pRS424TEF This study
Strains
W303A-pY26 As in W303-1A and blank pY26TEF-GPD This study
W303A-RHO1 As in W303-1A and pY26-RHO1 This study
W303A-FKS2 As in W303-1A and pY26-FKS2 This study
Δalg3Δoch1-RHO1 As in Δalg3Δoch1 and pY26-RHO1 This study
Δalg3Δoch1-FKS2 As in Δalg3Δoch1 and pY26-FKS2 This study
Δalg3Δoch1-pY26 As in Δalg3Δoch1 and blank pY26TEF-GPD This study
Δalg3Δoch1-LYZ As in Δalg3Δoch1 and pRS424TEF-LYZ This study
W303A-pY26-LYZ(R59S) As in W303A with blank pY26TEF-GPD and pRS424TEF-LYZ (R59S) This study
Δalg3Δoch1-LYZ(R59S) As in Δalg3Δoch1 and pRS424TEF-LYZ (R59S) This study
Δalg3Δoch1-pY26-LYZ (R59S) As in Δalg3Δoch1 with pRS424TEF-LYZ (R59S) and blank pY26TEF-GPD This study
Δalg3Δoch1-RHO1-LYZ (R59S) As in Δalg3Δoch1 with pRS424TEF-LYZ (R59S) and pY26-RHO1 This study