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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae

Fig. 6

Secreted and N-glycosylated human lysozyme was increased by RHO1 overexpression. a Analysis of human lysozyme secretion by western blotting. 5 ml of cells were cultured in SD-UT (lacking uracil and tryptophan) liquid medium for 48 h and transferred into 50 mL fresh SD-UT (30 °C, 230 rpm) for 24 h. The secreted human lysozyme was collected from 30 ml of each supernatant and concentrated as described in the “Methods” section. The concentrated supernatant was analyzed by Western blotting with anti-FLAG antibody. 2Δ: Δalg3Δoch1. b Analysis of the relative content of human lysozyme. The ratios of human lysozyme were calculated by ImageQuant™ Software version 7.0. The relative content of lysozyme in the lane of Δalg3Δoch1-LYZ was defined to be 1 ± 0.03. White glycosylated human lysozyme; Grey non-glycosylated human lysozyme

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Microbial Cell Factories

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