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Table 1 Kinetic constants of 6h-Aadh2p synthesized in A. adeninivorans G1212/YIC102-6H-AADH2 for different substrates

From: Aadh2p: an Arxula adeninivorans alcohol dehydrogenase involved in the first step of the 1-butanol degradation pathway

Substrate KM [mM] kcat [1/s] kcat/KM [1/mM/s]
Ethanola 14.2 1419 99.9
Acetaldehydeb 26.5 15.2 0.6
1-Butanola 0.3 1312 4373
Butyraldehydec 0.5 11872 23744
NAD+d 0.5 2.2 4
NADHe 0.07 14.7 224.5
  1. aNAD+ was used in a concentration of 1 mM
  2. bNADH was used in a concentration of 0.8 mM
  3. cNADH was used in a concentration of 0.4 mM
  4. dEthanol was used in a concentration of 100 mM
  5. eAcetaldehyde was used in a concentration of 20 mM