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Table 1 Kinetic constants of 6h-Aadh2p synthesized in A. adeninivorans G1212/YIC102-6H-AADH2 for different substrates

From: Aadh2p: an Arxula adeninivorans alcohol dehydrogenase involved in the first step of the 1-butanol degradation pathway

Substrate

KM [mM]

kcat [1/s]

kcat/KM [1/mM/s]

Ethanola

14.2

1419

99.9

Acetaldehydeb

26.5

15.2

0.6

1-Butanola

0.3

1312

4373

Butyraldehydec

0.5

11872

23744

NAD+d

0.5

2.2

4

NADHe

0.07

14.7

224.5

  1. aNAD+ was used in a concentration of 1 mM
  2. bNADH was used in a concentration of 0.8 mM
  3. cNADH was used in a concentration of 0.4 mM
  4. dEthanol was used in a concentration of 100 mM
  5. eAcetaldehyde was used in a concentration of 20 mM
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Microbial Cell Factories

ISSN: 1475-2859

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