Fig. 5
From: Dissection of exopolysaccharide biosynthesis in Kozakia baliensis
Overview of additional EPS forming enzymes and clusters in K. baliensis. Figure (a) shows the levansucrase genes (ls) (EC 2.4.1.10) of K. baliensis DSM 14400 and NBRC 16680, flanked by a cysteine desulfurase (EC 2.8.1.7) (cd) and a ferric uptake regulation protein (fur), respectively. In (b) the genetic organization of the chromosomally located pol cluster of K. baliensis DSM 14400 is exemplarily depicted. The pol cluster contains 5 genes (polABCDE), which are functionally designated as polA: dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), polB: glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), polC: dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), polD: dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), polE: alpha-L-Rha alpha-1,3-l-rhamnosyltransferase (EC 2.4.1.-). c Shows an additional plasmid located levansucrase gene (EC 2.4.1.10) of K. baliensis DSM 14400. It shares highest similarity to Ga. diazotrophicus levansucrase lsdA and is flanked by a (partial) type II dependent secretion operon [45]). In (d) is the genetic organization of the K. baliensis DSM 14400 cellulose synthase operon illustrated. The operon consists of six genes: dgc/pd diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), dgc diguanylate cyclase, bcsZ endoglucanase precursor (EC 3.2.1.4), bcsQ NTPase, bcsAB cellulose synthase catalytic subunit [UDP-forming] (EC 2.4.1.12), bcsC cellulose synthase operon protein C