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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Display of a β-mannanase and a chitosanase on the cell surface of Lactobacillus plantarum towards the development of whole-cell biocatalysts

Fig. 5

Formation of manno-oligosaccharides (MOS) from LBG by mannanase-displaying L. plantarum cells. The picture shows HPAEC chromatograms of supernatants from the activity assay displayed in Fig. 4 (freshly harvested cells). (A) pSIP_ManBcwa2; (B) pSIP_1261ManB; (C) Standards: mannose, M1, mannobiose, M2, mannotriose, M3, mannohexaose, M6; (D) empty vector, pEV as negative control. Note that degradation of LBG galactomannan by a mannanase is expected to yield a wide variety of products (for which there are no standards), as is indeed observed in traces (A) and (B)

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Microbial Cell Factories

ISSN: 1475-2859

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