Fig. 8
Characteristic growth and enzyme production parameters of T. reesei Rut-C30 using miscanthus steepwater. T. reesei Rut-C30 was grown on 5 g L−1 glucose and 30 g L−1 α-cellulose containing 10, 20, 30, 50, 80, 100 % (v/v) of miscanthus steepwater. a Oxygen transfer rate (OTR); b cumulative oxygen transfer (OT). For clarity only every second measuring point over time is represented by a symbol; c filter paper activity. A asterisk marks a significant increase in filter paper activity compared to a culture without additions with significance level of 95 %, two asterisks correspond to a level of 99 %. Error bars represent standard deviation of technical triplicates. The start of the time axes was set to 12 h to increase the visibility of the results. Culture conditions: modified Pakula medium, 250 mL flask, filling volume 20 mL, shaking frequency 350 rpm, shaking diameter 50 mm, inoculum 106 spores mL−1, and 30 °C