Fig. 3

Characteristic growth and enzyme production parameters of T. reesei Rut-C30 on different types of cellulose. T. reesei Rut-C30 was grown on 5 g L⁻¹ glucose and 30 g L⁻¹ Sigmacell 101 (amorphous)/α-cellulose (Crl 41.5 %)/Sigmacell 20 (Crl 52.6 %)/Sigmacell 50 (Crl 56.1 %). a Oxygen transfer rate (OTR) and protein content in the culture supernatant. The OTR was measured in duplicates with a maximum mean error of 0.4 mmol L⁻¹ h⁻¹. For clarity of the depicted average values, only every second measuring point of OTR over time is represented by a symbol; b pH and filter paper activity. Error bars represent standard deviation of technical triplicates. Culture conditions: modified Pakula medium, 250 mL flask, filling volume 20 mL, shaking frequency 350 rpm, shaking diameter 50 mm, inoculum 10⁶ spores mL⁻¹, and 30 °C