Table 4 Deracemization of 2-hydroxy acids with recombinant E. coli coexpressing (S)-2-HADH, (R)-2-KAR and GDH
Entry | Substrate | Reaction time (h) | Conversion (%) | ee of (R)-1 (%) |
|---|---|---|---|---|
1 | rac-1a | 2 | 95.2 | >99 |
2 | rac-1b | 2 | 92.7 | >99 |
3 | rac-1c | 2 | 95.8 | >99 |
4 | rac-1d | 4 | 95.9 | >99 |
5 | rac-1e | 2 | 98.5 | >99 |
6 | rac-1f | 2 | 98.2 | >99 |
7 | rac-1g | 2 | 97.4 | >99 |
8 | rac-1h | 2 | 97.7 | >99 |
9 | rac-1i | 2 | 96.6 | >99 |
10 | rac-1j | 2 | 97.1 | >99 |
11 | rac-1k | 2 | 97.4 | >99 |
12 | rac-1l | 2 | 96.9 | >99 |
13 | rac-1m | 2 | 97.7 | >99 |
14 | rac-1n | 6 | 37.4 | 91.5 |
15 | rac-1o | 6 | 33.9 | 73.6 |
16 | rac-1p | 6 | 76.9 | >99 |
17 | rac-1q | 6 | 40.5 | >99 |
18 | rac-1r | 6 | 90.7 | 85.8 |
19 | rac-1s | 6 | 82.4 | 78.6 |