Fig. 1From: Enantioselective cascade biocatalysis for deracemization of 2-hydroxy acids using a three-enzyme systemTime course of deracemization of rac-1a with the mixtures of recombinant E. coli strain. The freshly prepared cells of E. coli BL21(DE3)/pET28b-HADH and E. coli BL21(DE3)/pCDFDuet-KAR-GDH were mixed in 10 mL of phosphate buffer (100 mM, pH 7.5) to a cell density of 4 and 8 g DCW/L, respectivelyBack to article page