Characterization of the aurantimycin biosynthetic gene cluster and enhancing its production by manipulating two pathway-specific activators in Streptomyces aurantiacus JA 4570

Zhao, Houyuan; Wang, Liang; Wan, Dan; Qi, Jianzhao; Gong, Rong; Deng, Zixin; Chen, Wenqing

doi:10.1186/s12934-016-0559-7

Table 1 Deduced functions for the orfs in ATM biosynthetic gene cluster

From: Characterization of the aurantimycin biosynthetic gene cluster and enhancing its production by manipulating two pathway-specific activators in Streptomyces aurantiacus JA 4570

Protein	Size^a	Accession no.	Proposed function	Homolog. origin	Identity/similarity
Orf1	206	EFE72573	Sugar transporter	SSFG_07808, Streptomyces ghanaensis ATCC 14672	82/88
Orf2	91	EME58614	Phosphopantetheine attachment site domain-containing protein	H074_18583, Amycolatopsis decaplanina DSM 44594	35/65
Orf3	181	AKJ15912	NADH: riboflavin 5′-phosphate oxidoreductase	ABB07_39600, Streptomyces incarnatus	57/67
Orf4	393	EFE72565	Cytochrome P450 hydroxylase	SSFG_07800, Streptomyces ghanaensis ATCC 14672	76/88
Orf5	378	KTF47283	Zinc-binding dehydrogenase	APS67_01215, Streptomyces sp. AVP053U2	82/90
Orf6	312	EFE73053	3-Oxoacyl-[acyl-carrier-protein] synthase III	SSGG_00419, Streptomyces roseosporus NRRL 15998	71/82
Orf7	82	KMO67717	Phosphopantetheine attachment site domain-containing protein	MCHLDSM_06970, Mycobacterium chlorophenolicum	38/55
Orf8	77	XP_008613423	Dihydrolipoamide succinyltransferase	SDRG_09281, Saprolegnia diclina VS20	44/62
Orf9	326	KOX10942	2-Oxoisovalerate dehydrogenase	ADK66_07235, Micromonospora sp. NRRL B-16802	50/64
Orf10	279	KTF47288	3-Methyl-2-oxobutanoate dehydrogenase	APS67_01220, Streptomyces sp. AVP053U2	86/91
ArtA	71	KOG32626	MbtH-like protein	ADK37_26530, Streptomyces resistomycificus	87/94
ArtB	240	KUN90566	Putative regulator	AQJ84_39630, Streptomyces resistomycificus	77/86
ArtC	530	KUM93570	A	AQI88_26200, Streptomyces cellostaticus	84/90
ArtD	77	AKJ15824	PCP	ABB07_39115, Streptomyces incarnatus	84/90
ArtE	395	KUM93571	FAD-dependent oxidoreductase	AQI88_26210, Streptomyces cellostaticus	81/90
ArtF	2882	KUM93572	C-A-PCP-E-C-A-MT-PCP	AQI88_26215, Streptomyces cellostaticus	81/88
ArtG	2574	KOU40387	C-A-PCP-E-C-A-PCP	ADK54_22435, Streptomyces sp. WM6378	78/86
ArtH	1274	KJY47954	C-A-PCP-TE	VR46_00735, Streptomyces sp. NRRL S-444	78/87
ArtI	248	KUM93575	TE	AQI88_26230, Streptomyces cellostaticus	86/90
ArtJ	312	KOG32633	ABC transporter	ADK37_26575, Streptomyces resistomycificus	86/92
ArtK	253	KIF05972	ABC transporter permease	PL81_10120, Streptomyces sp. RSD-27	81/92
ArtL	73	KUM93581	PCP	AQI88_26270, Streptomyces cellostaticus	86/90
ArtM	416	KUM99154	Cytochrome	AQI95_40375, Streptomyces yokosukanensis	89/93
ArtN	245	KUM93583	TE	AQI88_26280, Streptomyces cellostaticus	79/87
ArtO	1034	KUM93584	KS-AT-ACP	AQI88_26285, Streptomyces cellostaticus	80/86
ArtP	1899	KUM93585	KS-AT-DH-KR-ACP	AQI88_26290, Streptomyces cellostaticus	71/79
ArtQ	2199	KUM93586	KS-AT-DH-ER-KR-ACP	AQI88_26295, Streptomyces cellostaticus	77/85
ArtR	1040	KJY47935	KS-AT-ACP	VR46_00775, Streptomyces sp. NRRL S-444	81/89
ArtT	1061	KUM93588	C-A-PCP	AQI88_26305, Streptomyces cellostaticus	72/81
ArtU	248	AGK78157	DUF1838	SFUL_3223, Streptomyces fulvissimus DSM 40593	89/93
ArtV	260	KUL64059	Peptidoglycan-binding protein	ADL30_00970, Streptomyces sp. NRRL S-1521	77/83
ArtW	116	CAJ88026	Conserved hypothetical protein	SAMR0316, Streptomyces ambofaciens ATCC 23877	78/82
ArtX	209	KOT40573	TetR family Transcriptional regulator	ADK41_12650, Streptomyces caelestis	90/93
Orf11	467	AJE84472	Integrin-like protein	SLNWT_4096, Streptomyces albus	82/88
Orf12	261	AGM04325	Putative ArsR family transcriptional regulator	AORI_1737, Amycolatopsis orientalis HCCB10007	45/60
Orf13	176	AKJ08646	Hypothetical protein ABB07_00890	ABB07_00890, Streptomyces incarnatus	49/62

Orf1(STRAU_0320)…………Orf12(STRAU_0357)
^aNumbers are in amino acids

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ISSN: 1475-2859

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