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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Characterization of a pathway-specific activator of milbemycin biosynthesis and improved milbemycin production by its overexpression in Streptomyces bingchenggensis

Fig. 5

Effect of site-directed mutation in the AAA domain of MilR on its function. a Milbemycin production in the complementation strains containing the WT and mutant MilRs. MilR:ΔmilR/milR, G31A:ΔmilR/G31A, G32A:ΔmilR/G32A, G34A:ΔmilR/G34A, G36A:ΔmilR/G36A, K37A:ΔmilR/K37A, K37R:ΔmilR/K37R, S38A:ΔmilR/S38A, D122A:ΔmilR/D122A, D123A:ΔmilR/D123A. b Western blotting analysis of the WT and several selected mutant MilRs. The proteins of MilR and mutant MilRs (G31A, K37A, S38A, D122A and D123A) showed similar expression levels. c Transcriptional analysis of milR, milA4, milE and milF by semiquantitative RT-PCR. hrdB transcript was used as an internal control. MilR:ΔmilR/milR, 31A:ΔmilR/G31A, 32A:ΔmilR/G32A, 34A:ΔmilR/G34A, 36A:ΔmilR/G36A, 37A:ΔmilR/K37A, 37R:ΔmilR/K37R, 38A:ΔmilR/S38A, 122A:ΔmilR/D122A, 123A:ΔmilR/D123A; *P < 0.05

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