Fig. 5

Fluorescence intensity profiles (Fl. Int.; scale in arbitrary units) of wild type and mutant spores adsorbed with mRFP. The profiles were generated from fluorescence microscopy images using the 3D Surface plotter function of Image J (http://imagej.nih.gov/ij/). a Representative fluorescence intensity profiles of a wild type (left) and cotH mutant spore (right). The fluorescence intensity is reported in arbitrary units. b Box plots displaying the total corrected cellular fluorescence (TCCF) for 80 different spores of each strain. Limits of each box represent the first and the third quartile (25 and 75 %) and the values outside the boxes represent the maximum and the minimum values. The line dividing the box indicates the median value for each strain. P value is less than 0.0001