Fig. 3

(a) Dot blot of released mRFP after three washes (W1, W2 and W3) with PBS buffer at pH 3 or pH 7 or after extraction with 1 M NaCl, 0.1 % Triton X-100 (Ext) (“Methods” section). Purified recombinant mRFP (mRFP) and unbound mRFP (u-mRFP) after the adsorption reaction were used as markers. b Kinetic of mRFP release based on the densitometric analysis (Additional file 4: Table S3) of mRFP released upon incubation either in PBS buffer pH 7.0 (triangles) or 1 M NaCl, 0.1 % Triton X-100 (squares). c Western blot of mRFP extracted by a SDS–DTT or after consecutive SDS–DTT and urea treatments of wild type and cotH mutant spores. Immuno reactions were performed with anti-His primary antibody anti-His primary antibody conjugated with the horseradish peroxidase (“Methods” section)