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Fig. 7 | Microbial Cell Factories

Fig. 7

From: A β-glucosidase hyper-production Trichoderma reesei mutant reveals a potential role of cel3D in cellulase production

Fig. 7

a qRT-PCR analysis of the transcript abundance of genes encoding β-glucosidases and transcriptional factors in T. reesei TRB1 and RUT-C30 grown on 2 % microcrystalline cellulose for 5 days. b Gene clone of cel3D in strain RUT-C30 and the recombinant strains: TRB1, TRB2, TRB3 and TRB4. M: DNA marker; c Gene clone of cel3D, cel7B and cel3B in strain Rut-C30 and TRB1; d qRT-PCR analysis of the transcript abundance of gene cel3D in strain Rut-C30 and the recombinant strains: TRB1, TRB2, TRB3 and TRB4. e qRT-PCR analysis of the transcript abundance of cellulase gene expression in strain RUT-C30 and TRB1. The values show the mean of three replicates, and the error bar indicates the standard deviation, though in some cases they are too small to see on the graphical scale being used

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