Skip to main content

Table 1 Bacterial strains and plasmids used in this work

From: Lactococcus lactis carrying the pValac eukaryotic expression vector coding for IL-4 reduces chemically-induced intestinal inflammation by increasing the levels of IL-10-producing regulatory cells

  Characteristics Source
Strain
Escherichia coli TOP10 E. coli K-12-derived strain; F- mrcA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA 1 λ Life Technologies; Carlsbad, CA/USA
Escherichia coli TG1 E. coli K-12-derived strain; F’ [traD36 proAB + lacI q lacZΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (r K m K ) Lucigen; Middleton, MI/USA
Lactococcus lactis MG1363 (pValac::dts) L. lactis MG1363 strain carrying the pValac::dts plasmid This work
Lactococcus lactis MG1363 (pValac::dts::IL-4) L. lactis MG1363 strain carrying the pValac::dts::IL-4 plasmid This work
Lactococcus lactis MG1363 FnBPA+ L. lactis MG1363 strain expressing S. aureus FnBPA [28]
Lactococcus lactis MG1363 FnBPA+ (pValac::dts) L. lactis MG1363 FnBPA+ strain carrying the pValac::dts plasmid This work
Lactococcus lactis MG1363 FnBPA+ (pValac::dts::IL-4) L. lactis MG1363 FnBPA+ strain carrying the pValac::dts::IL-4 plasmid This work
Plasmid
pUC57::IL-4 E. coli cloning vector (ApR; pMB1 ori; lacZ) containing the Il-4 ORF GenScript; Piscataway, NJ/USA
pCR™-Blunt E. coli cloning vector (KanR; ZeoR; pUC ori; Plac; lacZα; ccdB) Life Technologies; Carlsbad, CA/USA
pCR™-Blunt::IL-4 pCR™-Blunt containing the IL-4 ORF This work
pValac::dts Eukaryotic expression vector (pCMV; CmR; repA; repC) containing the SV40 DTS This work
pValac::dts::IL-4 pValac::dts containing the IL-4 ORF This work
  1. Ap R ampicillin resistance gene, pMB1 ori pMB1 origin of replication, IL-4 interleukin 4, ORF open reading frame, Kan R kanamycin resistance gene, Zeo R zeocin resistance gene, pUC ori pUC origin of replication, P lac lac promoter, pCMV cytomegalovirus promoter, Cm R chloramphenicol resistance gene, repA repA origin of replication, repC repC origin of replication, SV40 Simian virus 40, DTS DNA nuclear targeting sequence, gfp green fluorescent protein