Table 1 Bacterial strains and plasmids used in this work
Characteristics | Source | |
|---|---|---|
Strain | ||
Escherichia coli TOP10 | E. coli K-12-derived strain; F- mrcA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA 1 λ− | Life Technologies; Carlsbad, CA/USA |
Escherichia coli TG1 | E. coli K-12-derived strain; F’ [traD36 proAB + lacI q lacZΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (r −K m −K ) | Lucigen; Middleton, MI/USA |
Lactococcus lactis MG1363 (pValac::dts) | L. lactis MG1363 strain carrying the pValac::dts plasmid | This work |
Lactococcus lactis MG1363 (pValac::dts::IL-4) | L. lactis MG1363 strain carrying the pValac::dts::IL-4 plasmid | This work |
Lactococcus lactis MG1363 FnBPA+ | L. lactis MG1363 strain expressing S. aureus FnBPA | [28] |
Lactococcus lactis MG1363 FnBPA+ (pValac::dts) | L. lactis MG1363 FnBPA+ strain carrying the pValac::dts plasmid | This work |
Lactococcus lactis MG1363 FnBPA+ (pValac::dts::IL-4) | L. lactis MG1363 FnBPA+ strain carrying the pValac::dts::IL-4 plasmid | This work |
Plasmid | ||
pUC57::IL-4 | E. coli cloning vector (ApR; pMB1 ori; lacZ) containing the Il-4 ORF | GenScript; Piscataway, NJ/USA |
pCR™-Blunt | E. coli cloning vector (KanR; ZeoR; pUC ori; Plac; lacZα; ccdB) | Life Technologies; Carlsbad, CA/USA |
pCR™-Blunt::IL-4 | pCR™-Blunt containing the IL-4 ORF | This work |
pValac::dts | Eukaryotic expression vector (pCMV; CmR; repA; repC) containing the SV40 DTS | This work |
pValac::dts::IL-4 | pValac::dts containing the IL-4 ORF | This work |