Table 2 Identification of differentially expressed protein spots from A. pullulans cells under different levels of nitrogen via proteomics analysis with 2D-GE and MALDI-TOF/TOF
Spot no. | Protein name | Putative function | pI | Mascot score | Coverage (%) | Mass (Da) | Fold change | E value |
|---|---|---|---|---|---|---|---|---|
A17 | Meiotic expression up regulated protein 14 | Cell differentiation | 5.44 | 70 | 18 | 39,244 | 4.93 | 0.0013 |
A18 | S-adenosylmethionine synthase | Amino acid metabolism | 5.75 | 128 | 12 | 43,115 | 1,000,000a | 1.9e−009 |
A46 | Pyruvate decarboxylase | Glycolytic pathway | 5.57 | 163 | 21 | 64,181 | 2.97 | 6e−013 |
A52 | Fructose-bisphosphate aldolase, class II | Carbohydrate metabolism | 5.41 | 282 | 22 | 39,637 | 2.24 | 7.5e−025 |
A56 | ADP/ATP carrier protein-like protein | Energy metabolism | 9.81 | 64 | 30 | 33,892 | 2.32 | 0.0045 |
A57 | Adenylate kinase | Energy metabolism | 8.84 | 87 | 17 | 30,980 | 8.36 | 2.6e−005 |
A62 | ADP/ATP carrier protein-like protein | Energy metabolism | 9.81 | 243 | 30 | 33,892 | 2.14 | 6e−021 |
A103 | 60S ribosomal protein L11 | Ribosome | 10.08 | 81 | 18 | 21,863 | 3.10 | 9.5e−005 |
A118 | Histone H2B | Nucleosome | 10.16 | 159 | 37 | 15,047 | 2.25 | 1.5e−012 |
B02 | Lysophospholipase Plb2 | Lipid metabolism | 4.31 | 44 | 2 | 71,790 | 1,000,000 | 0.00022 |
B11 | NADH-quinone oxido-reductase | Electron transport | 5.71 | 245 | 24 | 82,010 | 1.68 | 3.8e−021 |
B21 | Casein kinase II subunit beta | Cell differentiation | 4.40 | 122 | 7 | 38,463 | 2.01 | 7.5e−009 |
B24 | Nucleosome assembly protein-like protein | Cell differentiation | 4.26 | 299 | 25 | 45,383 | 1.97 | 1.5e−026 |
B41 | Glyceraldehyde-3-phosphate dehydrogenase | Glycolytic pathway | 6.25 | 260 | 21 | 36,436 | 2.40 | 1.2e−022 |