Table 4 Primers used in this work

P1

ATGCCTTTTACCCCGCTCCG

For gaaC amplification from A. niger genome, for colony PCR and sequencing (forward)

P2

CTAAGCAATATCCGGCAACG

For gaaC amplification from A. niger genome, for colony PCR and sequencing (reverse)

P3

CGGGGGATCCACTAGTTCTAGAGCGGCCGCGTGAGGGTCAGTTATTTCAT

For fragment 1 (−1000 bp HIS3 locus) amplification from S. cerevisiae (forward)

P4

TATTTCTTTCTACAAAAGCCCTCCTACCCATCTTTGCCTTCGTTTATCTTG

For fragment 1 (−1000 bp HIS3 locus) amplification from S. cerevisiae (reverse)

P5

TAACTCGAAAATTCTGCGTTCGTTAAAGCTAGCTGCAGCATACGATATAT

For fragment 4 (+1000 bp HIS3 locus) amplification from S. cerevisiae (forward)

P6

AAGCTGGAGCTCCACCGCGGTGGCGGCCGCGGAGCCATAATGACAGCAGT

For fragment 4 (+1000 bp HIS3 locus) amplification from S. cerevisiae (reverse)

P7

AATGAGCAGGCAAGATAAACGAAGGCAAAGATGGGTAGGAGGGCTTTTGT

For fragment 2 (his5) amplification from S. pombae (forward)

P8

TTCAGTTTTGGATAGATCAGTTAGAAAGCTATTAAGGGTTCTCGAGAGCT

For fragment 2 (his5) amplification from S. pombae (forward)

P9

GGAAGATATGATCTACGTATGGTCATTTCTTC

For TPI1/gaaD amplification from B5470 (forward)

P10

GGAGATCTCGAATTGGAGCTAGAGAAAG

For TPI1/gaaD amplification from B5470 (reverse)

P11

GATCTACGTATGGTCATTTCTTC

For colony PCR and sequencing gaaD ORF (forward)

P12

TCGAATTGGAGCTAGACAAAG

For colony PCR and sequencing gaaD ORF (reverse)

P13

ATGGCTCCCCCAGCTGTGTT

For colony PCR and sequencing gaaA ORF (forward)

P14

CTACTTCAGCTCCCACTTTC

For colony PCR and sequencing gaaA ORF (reverse)

P15

CCTCGCACCCATGTACATTGG

For colony PCR and sequencing gat1 ORF (forward)

P16

TTATATTGGCCTTTATGTCCGC

For colony PCR and sequencing gat1 ORF (reverse)

P17

TATATACCCGGGGTGCCACCTGACGTCTAAGA

For amplification of TPI1/gat1-gfp from B6367 (forward)

P18

TATATACCCGGGAGACCGAGATAGGGTTGAGT

For amplification of TPI1/gat1-gfp from B6367 (reverse)