Table 3 Plasmids used in this work

B1181

YEplac195 with PGK1 promoter, URA3, Amp ^R

pRS426

Yeast integration vector, URA3, Amp ^R

B5430

B1181 ligated to gaaC. Contains fragment 3 (PGK1/gaaC) of the gaaC integration vector in between bglII restriction sites

B5517

Yeast integration vector created by homologous recombination in yeast. Contains the gene gaaC under PGK1 promoter, the integration locus HIS3 and marker HIS5 (S. pombe) in between NotI restriction sites

B5470

pXY212 expressing the gene gaaD under TPI1 promoter

pRS405

Yeast integration vector, LEU2, Amp ^R

B5555

Yeast integration vector pRS405 expressing LEU2 and gaaD under TPI1 promoter in between AflII restriction sites

B2159

pXY212, containing TPI1 promoter region, Amp ^R

B5706

Genescript pUC57 plasmid containing codon-optimized gaaA in between EcoRI and BamHI restriction sites

B5696

B2159 ligated to gaaA under TPI promoter

pRS406

Yeast integration vector containing URA3, Amp ^R

B5697

Yeast integration vector containing URA3 and gaaA under TPI1 promoter in between AflII restriction sites

B3033

Yeast integration vector containing CAN1 locus, KnMX and lgd1 under PGK1 promoter in between LoxP sites and SaclL and Kpnl restriction sites

pSH66

Deletion vector containing Cre, Amp ^R

B6367

B2159 containing gat1-gfp under TPI1 promoter

B3531

Yeast integration vector containing KnMX marker, HO locus

B6382

B3531 yeast integration vector containing gat1-gfp under TPI1 promoter