Table 2 Strains used in this work

Escherichia coli TOP10

E. coli for electroporation hsdR, mcrA, lacZΔM15, recA

CEN.PK111-61A

S. cerevisiae CEN.PK 111-61A MATα, ura3-52, his3-Δ1, leu2-3112, TRP1, MAL28^c, SUC2

CEN.PK113-1A

S. cerevisiae CEN.PK 113-1A MATα, URA3, HIS3, LEU2, TRP1, MAL28^c, SUC2

S. cerevisiae ATCC 90845

MATα, his3Δ 200, ura3-52, leu2 Δ 1, lys2-Δ 202, trp1-Δ63

A. niger ATCC 1015

Template DNA for gene gaaC

Schizosaccharomyces pombe

Template DNA for gene his5

H4362

S. cerevisiae CEN.PK 111-61A+ gaaC (MATα, ura3-52, HIS5, leu2-3112, TRP1, MAL28^c, SUC2)

S. cerevisiae CEN.PK 111-61A

H4410

S. cerevisiae CEN.PK 111-61A + gaaC + gaaD (MATα, ura3-52, HIS5, LEU2, TRP1, MAL28^c, SUC2)

H4362

H4425

S. cerevisiae CEN.PK111-61A + gaaC + gaaD + gaaA (MATα, URA3, HIS5, LEU2, TRP1, MAL28^c, SUC2)

H4410

H4531

S. cerevisiae CEN.PK111-61A + gaaC + gaaD + gaaA + lgd1 (MATα, URA3, HIS5, LEU2, TRP1, MAL28^c, SUC2, can1⁻)

H4425

H4535

S. cerevisiae CEN.PK111-61A + gaaC + gaaD + gaaA + lgd1 + gat1-gfp (MATα, URA3, HIS5, LEU2, TRP1, MAL28^c, SUC2, can1⁻ , HO ⁻)

H4531