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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

Fig. 3

Flow cytometry analysis and hierarchial clustering of individual clones carrying episomal or integrative BFP-expressing vectors. a The overall BFP fluorescence signal was distributed between 10 equally spaced gates as percentage of fluorescence (being the total fluorescence for every clone equal to 100 %). Color-coded gating for frequencies of BFP fluorescence events in cultures derived from 40 individual clones expressing either integrative or episomal vectors (Green 0 %, Red 60 %). b Hierarchial clustering of fluorescence distribution; cluster 12 (containing 97.5 % of episomal clones, and 14, containing 45 % of integrative clones, are highlighted—see text and ‘‘Methods’’ section for details)

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Microbial Cell Factories

ISSN: 1475-2859

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