Fig. 1

Localization and purification of recombinant M.tb GAPDH. a Localization of rGAPDH in cellular fractions of M. smegmatis detection was done using α-GAPDH and α-His antibodies. b Localization of rGAPDH in cellular fractions of M.tb H37Ra detection was done using α-GAPDH and α-His antibodies. c Affinity purification of MS-GAPDH-H, analysis by 10 % SDS-PAGE. d Western blot with α-GAPDH and α-His to confirm purification from M. smegmatis. e Peptide mass fingerprinting (PMF) of 40 kD M. smegmatis protein that co-elutes with rGAPDH. Identified sequences are indicated in bold face. f Affinity purification of MT-GAPDH-H. g Western blot with α-GAPDH and α-His to confirm purification from M. tb H37Ra