Table 3 Strains and plasmids used in this study

Strains

 ZTK

W1485, ΔptsG ΔldhA ΔpflB; kan^r

Lab stock

 WSA110

ZTK, ΔlacZ::P _trc -zwf243-gnd361 from C. glutamicum; kan^r

This study

 WSA134

WSA110, P _BJ23100 -talB; P _BJ23100 -tktA; P _BJ23100 -pgl; kan^r

This study

 WSA138

WSA134, P _BJ23100 -pepck; Δppc::P _BJ23100 -pepck from A. succinogenes; kan^r

This study

 WSA146

WSA138, P _BJ23100 -sthA; kan^r

This study

 WSA150

WSA146, ΔackA-pta; kan^r

This study

 WSA152

WSA150, P _BJ23100 -galp; kan^r

This study

 WSA157

WSA152, ΔlldD::P _BJ23100 -pyc from C. glutamicum; kan^r

This study

 WSA159

WSA157, P _BJ23100 -dcuB; kan^r

This study

 WSA161

WSA157, P _BJ23100 -dcuC; kan^r

This study

 WSA163

WSA157, P _BJ23100 -dcuB; P _BJ23100 -dcuC; kan^r

This study

 WSA165

WSA159, Δzwf243-gnd361-protein bind; kan^r

This study

 WSA167

WSA163, Δzwf243-gnd361-protein bind; kan^r

This study

 DH5α

Cloning host

Lab stock

Plasmids

 pZY02E

p15 ori; Ptrc-zwf-gnd of E.coli; Cm^r

This study

 pZY02

p15 ori; Ptrc-zwf243-gnd361 of C. glutamicum; Cm^r

Lab stock

 pCU18

pBR322 ori; Amp^r

Lab stock

 pCU18-zwf243-gnd361

Ptrc-zwf243-gnd361 from pZY02, cat gene from pEL04, upstream and downstream fragments of lacz gene were cloned into pCU18; Amp^r, Cm^r

This study

 pCU18-pyc

P _BJ23100- pyc from C. glutamicum was cloned into pCU18; Amp^r

This study

 pCU18-ppc

Upstream and downstream fragments of ppc gene were cloned into pCU18; Amp^r

This study

 pCU18-cat-sacB

cat-sacB genes from pEL04 were cloned into pCU18-ppc; Amp^r, Cm^r

This study

 pCU18-pepck

P _BJ23100- pepck from A. succinogenes was cloned into pCU18-ppc; Amp^r

This study

 pEL04

cat-sacB cassette; Cm^r

[41]

 PTKSS

p15A replication, Tet^r, I-SceI restriction sites; Cm^r, Tet^r

[49]

 PTKRED

pSC10 replication, temperature sensitive replication origin, ParaBAD-driven I-SceI gene, red recombinase expression plasmid, lac-inducible expression; Spc^r

[49]