High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli

Table 2 Comparison of molar yields of succinate in anaerobic fermentation

Strains	Yield of succinate (mol/mol)	Increased yield compared with ZTK (%)	Yield compared with the theoretical maximum yield (%)
ZTK	1.01 ± 0.006	0	59
WSA134	1.21 ± 0.002	20	71
WSA146	1.31 ± 0.005	30	76
WSA157	1.40 ± 0.002	39	82
WSA159	1.53 ± 0.006	51	89
WSA163	1.54 ± 0.010	52	90
WSA165	1.50 ± 0.005	49	88

Bacteria were cultivated with 50 mL NBS medium in a 100 mL flask shaking at 220 rpm and 37 °C in anaerobic conditions with an initial OD600 of 0.6. Data are average values and standard deviations of triplicate experiments

ISSN: 1475-2859